When Is TFF Used?

Tangential flow filtration is used in concentration and diafiltration processes.

Concentration – Increases the concentration of a solution by removing fluids while keeping the solute molecules. This process is done by selecting a filter significantly smaller than the solute molecules to allow for a higher retention of solute molecules.

Diafiltration – The separation of small and large particles, leaving the smaller particles behind without altering the overall concentration at the end by washing out the remaining salt with another buffer. This process can also reduce the concentration of a solution by washing out the remaining salts with water.

Specific applications of TFF include, but are not limited to:

Pre-filtration component WFI systems to remove endotoxins.

Recovering of bacterial cultures and viruses.

Removing PVA that is excreted from the excipient from nanoparticles during drug loading.