Purification Filter Tube
Purification filter tube is a membrane separation technique used to separate extremely small particles and dissolved molecules in fluids. The primary basis for separation is molecular size, although other factors such as molecule shape and charge can also play a role.
Product Introduction
1.Description
Purification filter tube is a membrane separation technique used to separate extremely small particles and dissolved molecules in fluids. The primary basis for separation is molecular size, although other factors such as molecule shape and charge can also play a role. Molecules larger than the membrane pores will be retained, but not bound, at the surface of the membrane (not in the polymer matrix as they are retained in microporous membranes) and concentrated during the ultrafiltration process.
Compared to non-membrane processes (chromatography, dialysis, solvent extraction, or centrifugation), ultrafiltration and purification filter tube:
Is gentler to the molecules being processed. Does not require an organic extraction which may denature labile proteins.
Maintains the ionic and pH conditions. Is fast and relatively inexpensive. Can be performed at low temperatures (for example, in the cold room).
Is very efficient and can simultaneously concentrate and purify molecules.
2.MWCO Selection for Protein Applications
| The membrane MWCO | Biomolecule Molecular weight |
3kd | 10-20kd |
5kd | 20-30kd |
10kd | 30-100kd |
30kd | 90-150kd |
50kd | 150-300kd |
100kd | 300-800kd |
300kd | 800-2000kd |
The retention properties of ultrafiltration membranes are expressed as Molecular Weight Cut-off (MWCO) and measured in Kilodaltons (kD). This value refers to the approximate molecular weight of a dilute globular solute (i.e., a typical protein) which is 90% retained by the membrane. However, a molecule’s shape can have a direct effect on its retention by a membrane. For example, linear molecules like DNA may find their way through pores that will retain a globular species of the same molecular weight.
Hangzhou Guidling Technology CO.,LTD is an innovative manufacturer focusing on providing solutions (clarification, separation and concentration, etc.) in the downstream field of biopharmaceutical.
Guidling Technology has successfully developed centrifugal filter devices, ultrafiltration & microfiltration cassettes and TFF device and system, depth filter modules, virus clearance filter , etc. which can fully meet the requirements of concentration and desalination, virus clearance, fermentation liquid clarification and other processes in biological products.
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