Your Professional Tangential Flow Filtration Devices Supplier
Hangzhou Guidling Technology Co., Ltd. is an innovative manufacturer established in 2015, specializing in downstream biopharmaceutical purification and separation solutions. We focus on clarification, concentration, and filtration processes for biological production. Our main products include ultrafiltration & microfiltration cassettes, TFF systems, centrifugal filter devices, depth filter modules, and virus removal filters. These products are widely used in antibodies, vaccines, diagnostics, blood products, and cell culture applications.
Stable Manufacturing Capability
We have a complete production system for filtration products, ensuring consistent quality and stable supply. Our manufacturing process is designed to meet industrial biopharmaceutical standards.
Continuous Product Development
We keep improving our filtration technologies and have developed multiple product series since 2015. This allows us to provide updated and efficient solutions for downstream purification needs.
Flexible Application Solutions
Our products are designed for different bioprocess stages, including clarification, concentration, and virus removal. This helps customers build more efficient and customized filtration workflows.
Reliable Cooperation Support
We focus on long-term cooperation with customers by providing responsive communication and technical assistance. Our aim is to support smooth project operation from selection to application.
Tangential flow filtration devices are the rapid and efficient method for seperation and purification of biomolecules. They can be applied to a wide range of biological fields such as immunology, protein,chemistry, molecular biology,biochemistry,and microbiology.
Parameter
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Reservoir O-ring |
EP Rubber |
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Magnetic Stir Bar |
PTFE coated |
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Gauge Wetted Parts |
316L stainless steel |
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Gauge Mounting Block |
Polypropylene |
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Luer Fittings |
Polypropylene and stainless steel |
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Tubing |
C-flex﹡ |
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Four-way Valves |
Polycarbonate body, polyethylene core |
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Drip Tray |
Urethane |
Ease of use: Pump, gauge, and tubing setup is simple (as in the picture below). Along with the sample in the reservoir, the system is fully operable.
Speed of results: TFF operates continuously (rather than in batches) and rapidly separates and purifies biomolecules.
Ability to multitask: Lab workers can concentrate and diafilter samples on the same filtration system.
Flexibility: TFF units can process samples as small as 10 mL and as large as several thousands of liters.
Cell Harvesting
The separation and recovery of cells from fermentation.
Lysate Clarification
The separation and recovery of target molecules from cell debris (membrane fragments) and intact cells following cell lysis.
Product Fractionation
Protein separation based on molecule size.
Concentration
The concentration of retained proteins by removing unwanted solvents and small molecules.
Diafiltration
The exchange of buffer solution to final buffer formulation for additional processing.
Workflow Steps for Tangential Flow Filtration Devices
System and Sample Preparation
Thorough preparation is essential to ensure a successful TFF process. Pre-filter your sample to remove particulate sand protect the membrane from clogging. Prime the system with buffer to eliminate air and stabilize the membrane.
Sample Loading
Careful sample loading sets the foundation for effective filtration. Introduce your clarified sample into the feed reservoir, ensuring even flow into the system. Start the pump at a low flow rate to prevent pressure surges and ensure proper circulation.
Filtration
Filtration is the heart of TFF, where the membrane separates biomolecules by size. Microfiltration membranes(≥ 0.1 microns) remove cells, debris, and large molecules while retaining proteins while ultrafiltration membranes (< 0.1 microns) concentrate proteins, retaining larger molecules and allowing smaller ones to pass.
Separation of Permeate and Retentate
As filtration progresses, the permeate, containing smaller molecules, flows through the filter, while the retentate, with larger molecules, recirculates into the feed reservoir. This continuous loop ensures maximum separation efficiency, effectively removing smaller molecules while preserving valuable biomolecules.
Diafiltration (Optional)
When additional purification or buffer exchange is required, diafiltration offers a powerful solution. Add fresh buffer to the feed reservoir while maintaining the system’s total volume. This step removes unwanted salts or exchanges buffers, leaving your target biomolecule concentrated and purified.
Product Recovery
Recovery is the final critical step in TFF, where your concentrated biomolecule is carefully collected. Use sterile tools and containers to preserve integrity and minimize contamination. For added safety and purity, pass the product through a sterilizing-grade filter before transferring it to storage or downstream processing.
Customizable Options
● Adaptable for diverse scales with cassette capacities including (but not limited to):
0.5 – 5 m2
2 – 20 m2
4 – 40 m2
6 – 60 m2
● Fully customizable hollow fiber capacities
● Hazardous location ratings (Cl.1, Div.1, Div.2; ATEX Zone 0, 1, 2)
Packaging and Shipping
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Packaging |
Inner Packaging: Sterile protective bag or shrink wrap Outer Packaging: Extra-thick cardboard carton |
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Shipping |
For small quantity or sample will be shipped by DHL, FEDEX ,TNT ( shipping days 3-4 days). For bigger quantity will be shipped by sea shipment to your pointed nearest seaport ( shipping days 1 month). |
Our Factory
Our factory is located in the Hangzhou City, we have 5 production lines, which can produce 8000pcs cassettes and 5000pcs holders, and have about 100 workers in the factory.
We're well-known as one of the leading tangential flow filtration devices manufacturers and suppliers in China. If you're going to wholesale bulk high quality tangential flow filtration devices in stock, welcome to get free sample from our factory.
