FAQs about Hollow Fiber
Key words: hollow fiber, biopharmaceuticals, cell culture, cell separation, purification, concentration, protein purification, depth filtration, protein concentration
Q1. In the field of biopharmaceuticals, what drug manufacturing processes can use hollow fibers?
Hollow fiber can be used in many drug manufacturing processes in the field of biopharmaceuticals, such as cell culture, separation and purification, protein purification, and depth filtration. They are usually used as a means of separating impurities, reducing contamination, increasing yield and increasing purity.
Q2. Which steps can use hollow fiber in gene therapy, cell therapy companies?
In gene therapy and cell therapy companies, hollow fibers can be used in these process, such as cell culture, cell separation, washing, concentration, purification, etc. Specific applications include:
Cell culture: hollow fibers can be used as support materials for cell culture, providing greater cell density and surface area.
Cell separation: hollow fibers can be used for cell isolation and collection, increasing the purity and productivity of individual cells
Washing and concentration: hollow fibers can be used for washing, protein concentration and cell preparation.
Purification: hollow fibers can be used in the purification steps of biological agents such as proteins, viruses, and plasmids to improve product purity and productivity.
Case applications include:
①When preparing cell preparations related to CAR-T therapy, use hollow fibers to concentrate, collect and purify T cells.
②Screen and purify the gene sequences through hollow fiber to prepare plasmids for gene therapy.
③Recombinant proteins were purified and concentrated using hollow fibers for the preparation of biological drugs.
Q3. Case study of hollow fiber used in the collection of bacteria
Hollow fibers are widely used in the collection of bacteria, the following is one of the case studies.
Cultivate and collect Rhodotorula strains, which grow slowly in culture medium and are difficult to collect by traditional methods such as centrifugation. The use of hollow fibers will quickly and conveniently collect the bacteria and replace them with the buffer we needed.
Q4. Hollow fibers are used for the process steps of polysaccharide concentration
Hollow fiber is a kind of material usually used for polysaccharide concentration, the following are the process steps for polysaccharides concentration using hollow fibers:
Preparing hollow fibers: Put the hollow fiber in deionized water to make it fully wet.
Install samples: The solution containing polysaccharide is poured into the hollow fiber, and the solution can flow into the hollow fiber through the pressure difference.
Process the samples: The polysaccharide solution in the hollow fiber can be processed, such as to remove unnecessary water and increase the concentration of polysaccharide by heating, evaporating, etc.
Collect concentrate: The concentrated polysaccharide solution is collected from the hollow fibers, the unnecessary water and other impurities can be removed by backwashing or using a small pressure difference.
Verify the concentration effect: Use appropriate methods (e.g. UV absorption spectroscopy, HPLC, etc.) to verify that the concentration of the polysaccharide after concentration is as expected.
Store concentrate: For the polysaccharide concentrates that have been verified as qualified, they can be stored under appropriate conditions for subsequent application research, etc.
