The Guidling Ultrafiltration Centrifugal Filter Devices | A Highly Efficient Concentrator in The Laboratory

Product advantage

● Optimized filter structure, greatly reduce concentration polarization

● Easy to operate, fast and efficient concentration and liquid change

● High concentration ratio, high sample recovery

 

Application field

● Concentration, desalting and buffer replacement of protein, antibody, enzyme, nucleic acid and other biological samples

● Purification of viruses, bacteriophages, exosomes, vesicles, microparticles, etc

● Drug screening

● Protein enrichment

● Small molecule removal

● Pretreatment of analytical samples

 

Technical parameter

● Maximum initial sample capacity: 15mL, typical concentration after the final volume of 200μL

● Ultrafiltration tube design: standard 50mL centrifugal tube

● Built-in filter membrane is polyether sulfone filter membrane

● Operating temperature range: 0-45℃

●pH range: 1-14

● Fixed Angle (35°) rotor: 5000×g; Hanging basket rotor: 4000×g

 

Aperture type selection

For proteins, it is recommended to select a membrane aperture 2-6 times smaller than the molecular weight of the target protein. If the flow rate is considered, it is recommended to select a membrane aperture lower than this range (2X-6X).

 

Ordering information

Typical concentration volume and centrifugation time

Centrifugation conditions: 5000×g, room temperature, initial volume of 12mL

Protein molecular weight standard: 3kd, 5kd, 10kd using cytochrome c; 30kd and 50kd use BSA; 100kd IgG; n=6 (average of 3 filter batches)

Typical retention rate of protein molecular weight standard