Why use tangential flow filtering
The device is simple, the dead volume is small, and the sample processing time is fast. It can be carried out quickly from research and development to production. Compared with centrifugal concentration or dead end filtration device, it can obtain higher concentration samples in a shorter time.
Perform two steps in the same system - concentrate and percolate the sample in the same system, saving time and avoiding product loss.
Low equipment investment, small floor area and low treatment cost - the membrane package used in the tangential flow filtration system can be used repeatedly or once; The product is easy to detect and the integrity test is simple to ensure that there are no defects in use. It is especially suitable for the production of high value-added biological products
Tangential flow filtration principle
Tangential flow filtration is also called "cross flow". The pressure drives the fluid to pass through the surface of the filter membrane and wash away the trapped molecules, which greatly reduces the concentration polarization; The solute and solvent molecules with pressure less than the pore size of the membrane are formed on both sides of the membrane, and those larger than the pore size of the membrane return to the original solution for multiple cycles to complete concentration and separation; In dead end filtration (DC filtration), the filtration pressure is increased, the concentration polarization is easy to form on the membrane surface, the separation speed is slow and the effect is poor; In contrast, in the tangential flow filtration process, the formation of the limiting layer is prevented by the recirculation of the mixture. This recirculation is similar to that the separation is not easy to gather on the membrane surface, and undergoes repeated scouring, resulting in a long service life of the membrane; Therefore, using tangential flow filtration to separate biomolecules such as protein, polysaccharide and blood products has higher efficiency and faster concentration and separation speed.
