The Application of Depth Filtration Technology in Biopharmaceuticals
The Application of Depth Filtration Technology in Biopharmaceuticals
1. Fermentation Broth Clarification
Generally, after the biological fermentation is completed, a rough separation will be performed on the feed liquid, but there are still many negatively charged impurities remaining in the supernatant after the rough separation, such as colloidal substances, some cell debris, etc. Therefore, further clarification should be carried out on the fermented liquid after rough filtration. This step can be completed by using the deep membrane products of Hangzhou Guidling Technology Co., Ltd. Guidling GS series deep membrane stack filter element uses fiber as the main raw material, and can effectively remove impurities and microorganisms in the liquid through mechanical interception and electrostatic adsorption. When completing the clarification of the fermentation broth, it can also protect the downstream equipment and prolong the service life of the filter element.
2. Depyrogenation
Pyrogens, also known as endotoxins, are cell debris that are produced on the outer wall of Gram-negative bacteria. As a lipopolysaccharide substance, it has millions of molecular weights. It should be known that the organism will be greatly harmed by pyrogens, so pyrogens must be removed during the production of biological products. Usually, the way of repeated adsorption of activated carbon can be used to remove pyrogens in the preparation, but this method does not have a good effect. Besides, it requires greater labor intensity and greater loss. However, using depth filters to remove heat can effectively solve these problems. They organically combine electrostatic adsorption and mechanical interception methods. They not only have a high product yield, but also have good product quality, and does not cause too much energy consumption.
3. Small molecule refining
In terms of small molecules of antibiotics, the traditional production process requires very complicated processes, such as filtration, concentration, crystallization and so on. There are many shortcomings in the traditional process, such as low harvest rate, high energy consumption, etc. Depth filtration technology can be used to remove macromolecular impurities and simplify the operation process. Compared with the traditional filtering method, this method has many advantages, including high efficiency, easy operation and so on.
4. Large molecule refining
With the continuous development of biotechnology, large molecule protein drugs are also increasing rapidly. In view of their thermal instability, the main refining method is low-temperature and rapid depth filtration technology. Depth filtration technology removes a large number of impurities and concentrates active ingredients through the control of precision.
5. Separation and purification of blood products and other biological products
The separation and purification method in blood products and other biological products is mainly depth filtration. Adsorption and progressive porosity of depth filtration can effectively remove viral proteins, such as lipids, a large amount of nucleic acid, and foreign proteins, in the production of blood products and other biological products. Therefore, the depth filtration technology with low pressure difference, high throughput, stable temperature and stable operation is widely used in blood products and biological products.
